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Transcriptome analysis shows ambiguous phenotypes of murine primitive endoderm‐related stem cell lines
Author(s) -
Zhong Yixiang,
Binas Bert
Publication year - 2019
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12678
Subject(s) - endoderm , biology , phenotype , transcriptome , wnt signaling pathway , microbiology and biotechnology , embryonic stem cell , stem cell , cell culture , cell , in vivo , genetics , gene , signal transduction , gene expression
Primitive endoderm (PrE)‐related cell lines (XEN, pXEN and nEnd cells) show key features of the PrE. By transcriptome analysis, we show: (a) Compared to embryonic stem cells, PrE‐related cell lines are less in vivo like, although early nEnd cells are most similar to the PrE. (b) These cell lines show post‐PrE features of parietal (XEN and pXEN cells) or visceral (nEnd cells) endoderm, likely driven by Tgf‐β and Wnt/Activin signaling, respectively. (c) pXEN and nEnd cell lines additionally show pre‐PrE features. Hence, neither pXEN nor nEnd cell cultures represent a distinct in vivo entity. Rather, their properties are compatible with mixed and hybrid phenotypes. Our findings indicate that pre‐PrE, PrE and early post‐PrE phenotypes result from different niches, which need to be better understood to derive cell lines that distinctly represent the early stages of the extraembryonic endoderm.