Premium
Degradation of phosphate polymer polyP enhances lactic fermentation in mice
Author(s) -
Nakamura Akihiro,
Kawano Natsuko,
Motomura Kei,
Kuroda Akio,
Sekiguchi Kiyoshi,
Miyado Mami,
Kang Woojin,
Miyamoto Yoshitaka,
Hanai Maito,
Iwai Maki,
Yamada Mitsutoshi,
Hamatani Toshio,
Saito Takakazu,
Saito Hidekazu,
Tanaka Mamoru,
Umezawa Akihiro,
Miyado Kenji
Publication year - 2018
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12639
Subject(s) - biology , polyphosphate , biochemistry , phosphate , lactic acid , adenosine triphosphate , fermentation , mitochondrion , hydrolysis , intracellular , atp hydrolysis , bacteria , enzyme , atpase , genetics
In bacteria, a polymer of inorganic phosphate (Pi) (inorganic polyphosphate; polyP) is enzymatically produced and consumed as an alternative phosphate donor for adenosine triphosphate (ATP) production to protect against nutrient starvation. In vertebrates, polyP has been dismissed as a “molecular fossil” due to the lack of any known physiological function. Here, we have explored its possible role by producing transgenic (TG) mice widely expressing Saccharomyces cerevisiae exopolyphosphatase 1 (ScPPX1), which catalyzes hydrolytic polyP degradation. TG mice were produced and displayed reduced mitochondrial respiration in muscles. In female TG mice, the blood concentration of lactic acid was enhanced, whereas ATP storage in liver and brain tissues was reduced significantly. Thus, we suggested that the elongation of polyP reduces the intracellular Pi concentration, suppresses anaerobic lactic acid production, and sustains mitochondrial respiration. Our results provide an insight into the physiological role of polyP in mammals, particularly in females.