Issue Information

Front cover: Several types of super‐resolution fluorescence microscopies have been invented and begun to spread. One of them is the stimulated emission depletion (STED) microscopy, and its principle is illustrated in this drawing. In the traditional fluorescence microscopy, it is difficult to observe in sub‐diffraction limit resolution, since there is a limit to reducing the spot size of excitation beam due to the diffraction limit. On the other hand, a STED microscope can narrow the excited area enough by overlapping the excitation beam and doughnut‐shape STED beam. In this drawing, the woman's two umbrellas emit the excitation beam (cyan) and STED beam (orange) overlapping each other, and the fluorescence (green) is emitted from the area much narrower than the excitation beam spot. Designed by TRAIS Co., Ltd. (Kobe, Japan).