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Contribution of neuraminidase 3 to the differentiation of induced regulatory T cells
Author(s) -
Kaminuma Osamu,
Katoh Shigeki,
Miyagi Taeko,
Watanabe Nobumasa,
Kitamura Noriko,
Nishimura Tomoe,
Saeki Mayumi,
Mori Akio,
Hiroi Takachika
Publication year - 2018
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12553
Subject(s) - biology , neuraminidase , downregulation and upregulation , foxp3 , sialic acid , microbiology and biotechnology , in vitro , messenger rna , cell culture , enzyme , immune system , gene , immunology , biochemistry , genetics
Neuraminidase family enzymes that hydrolyze the terminal sialic acid linkage in biomolecules are involved in various immune responses. We previously showed that Th1 and Th2 cells differentially express several neuraminidases. Herein, the expression of neuraminidases in induced regulatory T ( iT reg) cells was investigated in comparison with that in other T‐cell subsets. Contrary to the tendency toward higher neuraminidase 1 mRNA expression in in vitro‐differentiated Th2 cells, compared to Th1, Th17 and iT reg cells, we observed significantly higher expression of neuraminidase 3 (Neu3) in iT reg cells. Furthermore, the expression of Neu3 in FoxP3 + CD 62L − spleen cells was higher than that in FoxP3 + CD 62L + and FoxP3 − cells. Lentiviral expression of Neu3 in naïve CD 4 + T cells during the stimulation culture led to upregulation of FoxP3 expression. On the basis of these findings, we conclude that Neu3 contributes to the differentiation of iT reg cells by upregulation of FoxP3.