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Gene transfection of human mesenchymal stem cells with a nano‐hydroxyapatite–collagen scaffold containing DNA ‐functionalized calcium phosphate nanoparticles
Author(s) -
Tenkumo Taichi,
Vanegas Sáenz Juan Ramón,
Takada Yukyo,
Takahashi Masatoshi,
Rotan Olga,
Sokolova Viktoriya,
Epple Matthias,
Sasaki Keiichi
Publication year - 2016
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12374
Subject(s) - transfection , protamine , mesenchymal stem cell , viability assay , scaffold , microbiology and biotechnology , gene delivery , materials science , biology , cell , biochemistry , biomedical engineering , gene , medicine , heparin
This study aimed to fabricate a growth factor‐releasing biodegradable scaffold for tissue regeneration. We prepared multishell calcium phosphate (CaP) nanoparticles functionalized with DNA , polyethyleneimine ( PEI ), protamine and octa‐arginine (R8) and compared their respective transfection activity and cell viability measures using human mesenchymal stem cells. DNA –protamine complexes improved the transfection efficiency of CaP nanoparticles with the exception of those functionalized with R8. These complexes also greatly reduced the cytotoxicity of PEI . In addition, we also fabricated DNA –protamine‐functionalized CaP nanoparticle‐loaded nano‐hydroxyapatite–collagen scaffolds and investigated their gene transfection efficiencies. These experiments showed that the scaffolds were associated with moderate hMSC cell viability and were capable of releasing the BMP ‐2 protein into hMSC s following gene transfection. In particular, the scaffold loaded with protamine‐containing CaP nanoparticles showed the highest cell viability and transfection efficiency in hMSC s; thus, it might be suitable to serve as an efficient growth factor‐releasing scaffold.