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In the most popular type of next‐generation DNA sequencer, DNA fragments are PCR‐amplified in the slide's channel coated by a lawn of PCR primers to form myriads of clone DNA clusters. After that, the DNA sequence of each cluster is determined by taking photographs of fl uorescent signals on the slide with the image sensor again and again after each single‐nucleotide extension step of a novel dye‐terminator method. Thus, the reaction proceeding in the next‐generation sequencer is massively parallel sequencing. In this drawing, we schematically expressed this mechanism with a flying hawk (image sensor) viewing clusters of pine trees (DNA) on the channel‐shaped reclaimed land divided by embankments. Designed by TRAIS Co., Ltd. (Kobe, Japan)
Publication year - 2016
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12347
Subject(s) - dna sequencer , library , biology , dna sequencing , dna , clone (java method) , channel (broadcasting) , terminator (solar) , polymerase chain reaction , computational biology , genetics , microbiology and biotechnology , gene , computer science , physics , telecommunications , 16s ribosomal rna , ionosphere , astronomy