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Src family kinases suppress differentiation of brown adipocytes and browning of white adipocytes
Author(s) -
Usui Mai,
Uno Masaharu,
Nishida Eisuke
Publication year - 2016
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12340
Subject(s) - adipogenesis , white adipose tissue , biology , brown adipose tissue , thermogenesis , endocrinology , medicine , kinase , adipose tissue , prdm16 , browning , microbiology and biotechnology , biochemistry
Brown adipocytes and beige adipocytes can expend energy, generate heat, and increase whole‐body energy expenditure. The detailed mechanisms of adipogenesis and thermogenesis of these cells are still obscure. Here, we show that Src family kinases ( SFK s) regulate both brown adipogenesis and browning of white adipocytes. To identify factors involved in brown adipogenesis, we first examined the effect of several chemical inhibitors on the differentiation of brown preadipocytes isolated from mouse brown adipose tissue ( BAT ) and found that treatment with PP 2, the specific inhibitor of SFK s, promoted the differentiation. Another inhibitor of SFK s, PP 1, also promoted the brown adipogenesis, whereas an inactive analogue of PP 2, PP 3, did not. Moreover, over‐expression of C‐terminal Src kinase ( CSK ), the negative regulator of SFK s, also promoted brown adipogenesis. Next, we examined the effect of inhibition of SFK s on the differentiation of white preadipocytes isolated from white adipose tissue ( WAT ). Our results showed that either PP 2 treatment or CSK ‐over‐expression generated Ucp1‐positive beige adipocytes, thus inducing browning of white adipocytes. Finally, our analysis showed that the expression levels and activity of SFK s in WAT were much higher than in BAT . These results taken together suggest that SFK s regulate differentiation and browning of fat cells in vivo .