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Regulation of gene expression via retrotransposon insertions and the noncoding RNA 4.5S RNA H
Author(s) -
Ishida Kentaro,
Miyauchi Kenjyo,
Kimura Yuko,
Mito Mari,
Okada Shunpei,
Suzuki Tsutomu,
Nakagawa Shinichi
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12280
Subject(s) - biology , retrotransposon , antisense rna , rna , gene , gene expression , gene knockdown , oligonucleotide , reporter gene , microbiology and biotechnology , genome , genetics , transposable element
Short interspersed elements ( SINE s) comprise a significant portion of mammalian genomes and regulate gene expression through a variety of mechanisms. Here, we show that Myodonta clade‐specific 4.5S RNA H (4.5 SH ), an abundant nuclear noncoding RNA that is highly homologous to the retrotransposon SINE B1, controls the expression of reporter gene that contains the antisense insertion of SINE B1 via nuclear retention. The depletion of endogenous 4.5 SH with antisense oligonucleotides neutralizes the nuclear retention and changes the subcellular distribution of the reporter transcripts containing the antisense SINE B1 insertion. Importantly, endogenous transcripts with antisense SINE B1 were increased in the cytoplasm after knockdown of 4.5 SH , leading to a decrease in cellular growth. We propose a tentative hypothesis that the amplification of the 4.5 SH cluster in specific rodent species might delineate their evolutionary direction via the regulation of genes containing the antisense insertion of SINE B1.