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Role of 100S ribosomes in bacterial decay period
Author(s) -
Shcherbakova Ksenia,
Nakayama Hideki,
Shimamoto Nobuo
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12273
Subject(s) - ribosome , biology , green fluorescent protein , cytoplasm , ribosomal protein , escherichia coli , ribosomal rna , strain (injury) , microbiology and biotechnology , period (music) , gene , genetics , rna , anatomy , physics , acoustics
Ribosomal proteins S10 and S2 were each fused with GFP to track the fates of these proteins in the stationary growth phase and the following decay period in Escherichia coli . The fused proteins localized mainly in the cytoplasm, and their amounts were proportional to the colony‐forming unit. S10‐GFP strains that lacked genes responsible for regulating 100S ribosomes and S2‐GFP strain that was unable to form 100S both showed shortened stationary phases. This result indicates that these strains exhibit earlier death in the absence of 100S formation (S2‐GFP, S10‐GFP ∆rmf and S10‐GFP ∆hpf ) and breakdown (S10‐GFP ∆yfiA ). Therefore, in addition to the mere presence of 100S, the correct timing of 100S formation and breakdown is required to maintain viability. We propose a model in which 100S acts as a tentative repository of ribosomes that are protected from degradation and provide a source of amino acids in later growth period.