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Comprehensive analysis of sphingosine‐1‐phosphate receptor mutants during zebrafish embryogenesis
Author(s) -
Hisano Yu,
Inoue Asuka,
Taimatsu Kiyohito,
Ota Satoshi,
Ohga Rie,
Kotani Hirohito,
Muraki Michiko,
Aoki Junken,
Kawahara Atsuo
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12259
Subject(s) - biology , zebrafish , microbiology and biotechnology , mutant , maternal to zygotic transition , s1pr1 , genetics , sphingosine , embryogenesis , receptor , zygote , gene , embryo , cancer research , vascular endothelial growth factor a , vegf receptors , vascular endothelial growth factor
The lipid mediator sphingosine‐1‐phosphate (S1P) regulates various physiological and pathological phenomena such as angiogenesis and oncogenesis. Secreted S1P associates with the G‐protein‐coupled S1P receptors (S1PRs), leading to the activation of downstream signaling molecules. In mammals, five S1prs have been identified and the genetic disruption of a single S1pr1 gene causes vascular defects. In zebrafish, seven s1prs have been isolated. We found that individual s1prs showed unique expression patterns with some overlapping expression domains during early embryogenesis. We generated all s1pr single‐mutant zebrafish by introducing premature stop codons in their coding regions using transcription activator‐like effector nucleases and analyzed their phenotypes during early embryogenesis. Zygotic s1pr1 , s1pr3a , s1pr3b , s1pr4 , s1pr5a and s1pr5b mutants showed no developmental defects and grew into adults, whereas zygotic s1pr2 mutant showed embryonic lethality with a cardiac defect, showing quite distinct embryonic phenotypes for individual S1pr mutants between zebrafish and mouse. We further generated maternal‐zygotic s1pr1 , s1pr3a , s1pr3b , s1pr4 , s1pr5a and s1pr5b mutants and found that these maternal‐zygotic mutants also showed no obvious developmental defects, presumably suggesting the redundant functions of the S1P receptor‐mediated signaling in zebrafish.