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Stage‐specific reference genes significant for quantitative PCR during mouse retinal development
Author(s) -
Adachi Hiroko,
Tominaga Hiroyuki,
Maruyama Yuko,
Yoneda Kazuhito,
Maruyama Kazuichi,
Yoshii Kengo,
Kinoshita Shigeru,
Nakano Masakazu,
Tashiro Kei
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12254
Subject(s) - biology , reference genes , housekeeping gene , gene , retina , in silico , sdha , retinal , gene expression , real time polymerase chain reaction , genetics , computational biology , neuroscience , biochemistry
Developing mouse retina has been serving as an ideal model for investigating the molecular mechanism of neural development and angiogenesis, because several significant events associated with these physiological phenomena are drastically occurring in conjunction with retinal development. However, as many genes are influencing on each other to establish mature retina within 21 days from E10 to P12, we must carefully design the experiments, such as in the case of quantitating the amount of altered gene expression toward the establishment of retina by quantitative PCR . As we have seen considerable variations of quantitative results in different developmental stages of retina depending on the reference genes used for compensation, we here attempted to determine a reliable reference gene to accurately quantitate the target genes in each stage. According to the results of in silico prediction and comparison with a database of SAGE , we found that the most stable gene from early to late stages was S dha , whereas one of the most popular housekeeping genes, A ctb , was the one that could mislead the quantitative results even in the adult stage. Consequently, we pointed out the importance of selecting an appropriate reference gene, especially to quantitate the amount of gene expression in the developmental stages of a certain tissue.