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Regulation by gut commensal bacteria of carcinoembryonic antigen‐related cell adhesion molecule expression in the intestinal epithelium
Author(s) -
Kitamura Yasuaki,
Murata Yoji,
Park Jungha,
Kotani Takenori,
Imada Shinya,
Saito Yasuyuki,
Okazawa Hideki,
Azuma Takeshi,
Matozaki Takashi
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12247
Subject(s) - biology , butyrate , microbiology and biotechnology , carcinoembryonic antigen , intestinal epithelium , small intestine , cell adhesion molecule , messenger rna , intestinal mucosa , bacteria , epithelium , biochemistry , gene , medicine , genetics , cancer , fermentation
Carcinoembryonic antigen‐related cell adhesion molecule ( CEACAM ) 1 and CEACAM 20, immunoglobulin superfamily members, are predominantly expressed in intestinal epithelial cells ( IEC s) and co‐localized at the apical surface of these cells. We here showed that the expression of mouse CEACAM 1 and CEACAM 20 at both mRNA and protein levels was markedly reduced in IEC s of the small intestine by the treatment of mice with antibiotics against Gram‐positive bacteria. The expression of both proteins was also decreased in IEC s of the small intestine from germ‐free mice, compared with that from control specific‐pathogen‐free mice. Exposure of intestinal organoids to IFN ‐γ markedly increased the expression of either CEACAM 1 or CEACAM 20, whereas the exposure to TNF ‐α increased the expression of the former protein, but not that of the latter. In contrast, the expression of CEACAM 20, but not of CEACAM 1, in intestinal organoids was markedly increased by exposure to butyrate, a short‐chain fatty acid produced by bacterial fermentation in the intestine. Collectively, our results suggest that Gram‐positive bacteria promote the mRNA expression of CEACAM 1 or CEACAM 20 in the small intestine. Inflammatory cytokines or butyrate likely participates in such effects of commensal bacteria.