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Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas
Author(s) -
Nukaya Daiki,
Minami Kohtaro,
Hoshikawa Ritsuko,
Yokoi Norihide,
Seino Susumu
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12227
Subject(s) - biology , epigenetics , induced pluripotent stem cell , stem cell , gene , microbiology and biotechnology , gene expression , genetics , pancreas , embryonic stem cell , endocrinology
Induced pluripotent stem cells (i PSC s) have been established from various somatic cell types. Accumulating evidence suggests that i PSC s from different cell sources have distinct molecular and functional properties. Here, we establish i PSC derived from mouse pancreas (Panc‐i PSC ) and compared their properties with those of i PSC derived from tail‐tip fibroblast ( TTF ‐i PSC ). The metabolic profile differs between Panc‐i PSC and TTF ‐i PSC , indicating distinct cell properties in these i PSC s. Expression of Pdx1 , a marker of pancreas differentiation, is increased through formation of embryoid body ( EB ) in Panc‐i PSC , but the level is similar to that in TTF ‐i PSC . In contrast, EB s derived from Panc‐i PSC express liver‐specific albumin ( Alb ) and alpha‐fetoprotein ( Afp ) genes much more strongly than those from TTF ‐i PSC . Epigenetic analysis shows a different histone modification pattern between Panc‐i PSC and TTF ‐i PSC . Promoter regions of Alb and Afp genes in Panc‐i PSC are suggested to have a more open chromatin structure than those in TTF ‐i PSC , which also is seen in primary cultured pancreatic cells. Our data suggest that Panc‐i PSC possesses distinct differentiation capacity from that of TTF ‐ PSC , which may be influenced by epigenetic memory.