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Peroxin Pex14p is the key component for coordinated autophagic degradation of mammalian peroxisomes by direct binding to LC 3‐ II
Author(s) -
Jiang Li,
HaraKuge Sayuri,
Yamashita Shunichi,
Fujiki Yukio
Publication year - 2015
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12198
Subject(s) - biology , peroxisome , peroxisomal targeting signal , microbiology and biotechnology , biogenesis , mutant , biochemistry , receptor , gene
Pexophagy can be experimentally induced in mammalian cells by removing the culture serum. Pex14p, a peroxisomal membrane protein essential for matrix protein import in docking of soluble receptor Pex5p, is involved in the mammalian autophagic degradation of peroxisomes and interacts with the lipidated form of LC 3, termed LC 3‐ II , an essential factor for autophagosome formation, under the starvation condition in CHO ‐K1 cells. However, molecular mechanisms underlying the Pex14p‐ LC 3‐ II interaction remain largely unknown. To verify whether Pex14p directly binds LC 3‐ II , we reconstituted an in vitro conjugation system for synthesis of LC 3‐ II . We show here that Pex14p directly interacts with LC 3‐ II via the transmembrane domain of Pex14p. Pex5p competitively inhibited this interaction, implying that Pex14p preferentially binds to Pex5p under the nutrient‐rich condition. Moreover, a Pex5p mutant defective in PTS 1‐protein import lost its affinity for Pex14p under the condition of nutrient deprivation, thereby more likely explaining why Pex14p prefers to interact with LC 3‐ II under the starvation condition in vivo . Together, these results suggest that Pex14p is a unique factor that functions in the dual processes in peroxisomal biogenesis and degradation with the coordination of Pex5p in response to the environmental changes.