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Transcription cofactor PC 4 plays essential roles in collaboration with the small subunit of general transcription factor TFIIE
Author(s) -
Akimoto Yusuke,
Yamamoto Seiji,
Iida Satoshi,
Hirose Yutaka,
Tanaka Aki,
Hanaoka Fumio,
Ohkuma Yoshiaki
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12187
Subject(s) - transcription factor ii a , transcription factor ii h , transcription factor ii e , general transcription factor , transcription factor ii b , transcription preinitiation complex , transcription factor ii f , biology , microbiology and biotechnology , rna polymerase ii holoenzyme , transcription factor , transcription (linguistics) , promoter , genetics , transcriptional regulation , gene expression , gene , linguistics , philosophy
In eukaryotes, positive cofactor 4 (PC4) stimulates activator‐dependent transcription by facilitating transcription initiation and the transition from initiation to elongation. It also forms homodimers and binds to single‐stranded DNA and various transcriptional activators, including the general transcription factor TFIIH. In this study, we further investigated PC4 from Homo sapiens and the nematode Caenorhabditis elegans ( hPC 4 and cePC4, respectively). hPC 4 strongly stimulated transcription on a linearized template, whereas it alleviated transcription on a supercoiled template. Transcriptional stimulation by PC4 was also alleviated by increasing the amount of TFIID. GST pull‐down studies with general transcription factors indicated that both hPC 4 and cePC4 bind strongly to TFIIB, TFIIEβ, TFIIFα, TFIIFβ and TFIIH XPB subunits and weakly to TBP and TFIIH p62. However, only hPC 4 bound to CDK7. The effect of each PC4 on transcription was studied in combination with TFIIEβ. hPC 4 stimulated both basal and activated transcription, whereas cePC4 primarily stimulated activated transcription, especially in the presence of TFIIEβ from C. elegans . Finally, hPC 4 bound to the C‐terminal region of hTFIIE β adjacent to the basic region. These results indicate that PC4 plays essential roles in the transition step from transcription initiation to elongation by binding to melted DNA in collaboration with TFIIEβ.