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Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis
Author(s) -
Adachi Makoto,
Kawasaki Asami,
Nojima Hisashi,
Nishida Eisuke,
Tsukita Sachiko
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12179
Subject(s) - cytokinesis , iqgap1 , microbiology and biotechnology , biology , mitosis , ingression , cleavage furrow , cell division , cell , genetics , scaffold protein , signal transduction
IQGAP family proteins, comprising IQGAP 1, ‐2, and ‐3 in mammals, are involved in diverse ranges of cellular processes such as adhesion and migration. IQGAP proteins in yeast also play important roles in cytokinesis. However, the involvement of IQGAP proteins in cytokinesis in mammals remains unaddressed. In this study, we showed that IQGAP 3 specifically localized to the equatorial cortex at anaphase, whereas IQGAP 1 localized to the cell cortex uniformly and IQGAP 2 was unexpressed in HeLa cells. IQGAP 3, but neither IQGAP 1 nor ‐2, was able to interact with anillin, which was required for the localization of IQGAP 3 to the contractile ring. The suppressed expression of IQGAP 3 inhibited the completion of cleavage furrow ingression and led to the multinucleation of cells. The suppression of IQGAP 1 also had similar inhibitory effects on cytokinesis, and the simultaneous suppression of IQGAP 1 and ‐3 induced more severe effects. The localization of anillin and RhoA to the contractile ring was impaired by the suppression of IQGAP 1 and ‐3, whereas their upstream regulators, the centralspindlin complex and Ect2, remained unaffected. These results suggested that mammalian IQGAP proteins may play a role in cytokinesis by regulating the localization of key cytokinesis regulatory proteins to the contractile apparatus during mitosis.