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Transient expression of RAD 51 in the late G2‐phase is required for cell cycle progression in synchronous P hysarum cells
Author(s) -
Le Cigne Anthony,
MenilPhilippot Vanessa,
Fleury Fabrice,
Takahashi Masayuki,
Thiriet Christophe
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12174
Subject(s) - cell cycle , biology , mitosis , cell cycle progression , physarum polycephalum , microbiology and biotechnology , physarum , cell , homologous chromosome , genetics , gene
The homologous recombination factor RAD 51 is highly conserved. This criterion enabled us to identify a RAD 51 ortholog in P hysarum polycephalum . We found that the P hysarum protein presents a high homology to the human protein and cross‐reacted with antibodies directed against the human RAD 51. Taking advantage of the natural synchrony of millions of nuclei within a single cell of Physarum , we investigated the fluctuation of the amount of the Pp RAD 51 throughout the cell cycle. Our results showed that in the late G2‐phase, RAD 51 was transiently expressed in a large quantity. Furthermore, knocking‐down RAD 51 in the G2‐phase abolished this transient expression before mitosis and affected cell cycle progression. These results support the idea that RAD 51 plays a role in the progression of the cell cycle in the late G2‐phase.