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Formation of nuclear bodies by the lnc RNA Gomafu‐associating proteins Celf3 and SF 1
Author(s) -
Ishizuka Akira,
Hasegawa Yuko,
Ishida Kentaro,
Yanaka Kaori,
Nakagawa Shinichi
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12169
Subject(s) - biology , rna splicing , rna binding protein , rna , gene knockdown , nuclear protein , microbiology and biotechnology , alternative splicing , cell nucleus , gene , nucleus , messenger rna , genetics , transcription factor
Gomafu/ MIAT /Rncr2 is a long noncoding RNA that has been proposed to control retinal cell specification, stem cell differentiation and alternative splicing of schizophrenia‐related genes. However, how Gomafu controls these biological processes at the molecular level has remained largely unknown. In this study, we identified the RNA ‐binding protein Celf3 as a novel Gomafu‐associating protein. Knockdown of Celf3 led to the down‐regulation of Gomafu, and cross‐link RNA precipitation analysis confirmed specific binding between Celf3 and Gomafu. In the neuroblastoma cell line Neuro2A, Celf3 formed novel nuclear bodies (named CS bodies) that colocalized with SF 1, another Gomafu‐binding protein. Gomafu, however, was not enriched in the CS bodies; instead, it formed distinct nuclear bodies in separate regions in the nucleus. These observations suggest that Gomafu indirectly modulates the function of the splicing factors SF 1 and Celf3 by sequestering these proteins into separate nuclear bodies.