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Involvement of satellite I noncoding RNA in regulation of chromosome segregation
Author(s) -
Ideue Takashi,
Cho Yukiko,
Nishimura Kanako,
Tani Tokio
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12149
Subject(s) - biology , centromere , chromosome segregation , rna , microbiology and biotechnology , satellite , aurora b kinase , kinetochore , mitosis , gene knockdown , non coding rna , chromosome , genetics , gene , engineering , aerospace engineering
Human centromeres consist of repetitive sequences from which satellite I noncoding RNA s are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape‐shape phenotype. Co‐immunoprecipitation experiments showed that satellite I RNA associates with Aurora B , a component of the chromosome passenger complex ( CPC ) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP , another component of the CPC . In addition, depletion of satellite I RNA resulted in up‐regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.