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Absence of primary cilia in cell cycle‐arrested human breast cancer cells
Author(s) -
Nobutani Kentaro,
Shimono Yohei,
Yoshida Midori,
Mizutani Kiyohito,
Minami Akihiro,
Kono Seishi,
Mukohara Toru,
Yamasaki Takashi,
Itoh Tomoo,
Takao Shintaro,
Minami Hironobu,
Azuma Takeshi,
Takai Yoshimi
Publication year - 2014
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12122
Subject(s) - cilium , biology , cancer cell , breast cancer , cell cycle , cancer , pathology , cancer research , immunofluorescence , epithelium , microbiology and biotechnology , immunology , antibody , medicine , genetics
Previous studies using cultured cells showed that primary cilia are present in quiescent cells, but are absent in proliferating cells. We studied here the relationship between the presence or absence of primary cilia and the cell cycle arrest of normal epithelial cells and cancer cells in the human normal breast and breast cancer tissues. In normal breast tissues, although most epithelial cells were nonproliferating as estimated by the immunofluorescence staining of the proliferation marker Ki‐67, primary cilia were present only in 20–40% of the epithelial cells. In breast cancer tissues, primary cilia were not observed in any of the breast cancer cells. Furthermore, primary cilia were hardly observed in the nonproliferating cancer cells in the orthotopic and metastatic human breast cancer xenograft tumors in mice. These results indicate that the absence of primary cilia does not necessarily represent the proliferating phases of normal epithelial cells and cancer cells.