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Conservation of two distinct types of 100 S ribosome in bacteria
Author(s) -
Ueta Masami,
Wada Chieko,
Daifuku Takashi,
Sako Yoshihiko,
Bessho Yoshitaka,
Kitamura Aya,
Ohniwa Ryosuke L.,
Morikawa Kazuya,
Yoshida Hideji,
Kato Takayuki,
Miyata Tomoko,
Namba Keiichi,
Wada Akira
Publication year - 2013
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12057
Subject(s) - ribosome , biology , bacteria , 50s , escherichia coli , translation (biology) , protein biosynthesis , messenger rna , gene , biochemistry , biophysics , microbiology and biotechnology , genetics , rna
In bacteria, 70S ribosomes (consisting of 30S and 50S subunits) dimerize to form 100S ribosomes, which were first discovered in E scherichia coli . Ribosome modulation factor ( RMF ) and hibernation promoting factor ( HPF ) mediate this dimerization in stationary phase. The 100 S ribosome is translationally inactive, but it dissociates into two translationally active 70 S ribosomes after transfer from starvation to fresh medium. Therefore, the 100 S ribosome is called the ‘hibernating ribosome’. The gene encoding RMF is found widely throughout the G ammaproteobacteria class, but is not present in any other bacteria. In this study, 100 S ribosome formation in six species of G ammaproteobacteria and eight species belonging to other bacterial classes was compared. There were several marked differences between the two groups: (i) Formation of 100S ribosomes was mediated by RMF and short HPF in G ammaproteobacteria species, similar to E . coli , whereas it was mediated only by long HPF in the other bacterial species; (ii) RMF /short HPF ‐mediated 100 S ribosome formation occurred specifically in stationary phase, whereas long HPF ‐mediated 100 S ribosome formation occurred in all growth phases; and (iii) 100 S ribosomes formed by long HPF were much more stable than those formed by RMF and short HPF .