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Plasma membrane‐localized A l‐transporter from blue hydrangea sepals is a member of the anion permease family
Author(s) -
Negishi Takashi,
Oshima Kenshiro,
Hattori Masahira,
Yoshida Kumi
Publication year - 2013
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12041
Subject(s) - permease , sepal , biology , transporter , vacuole , arabidopsis , biochemistry , arabidopsis thaliana , ion transporter , solute carrier family , symporter , microbiology and biotechnology , botany , gene , membrane , mutant , pollen , stamen , cytoplasm
In hydrangea sepals, an aluminum complex of delphinidin‐3‐ O ‐glucoside is responsible for the development of the blue color, and co‐existing copigments mediate the solubilization and stabilization of the blue A l‐anthocyanin complex which is localized in the sepal vacuole. In addition, hydrangeas are A l‐hyperaccumulators and exhibit tolerance to acidic soils, in which the toxicity is due to soluble A l ion. Therefore, an A l‐absorbing transport and storage system must exist in hydrangea. Recently, we cloned vacuolar and plasma membrane‐localized A l‐transporters, H m VALT , and H m PALT 1, which are both members of the aquaporin family. However, H m PALT 1 was only expressed in the sepals, indicating that a different A l‐transporter should exist for absorption and long‐distance transportation in the hydrangea plant. Using genetic information and microarray analysis, we identified an additional aluminum transporter gene, HmPALT2 , which belongs to a member of the anion permease. The transcript was expressed in all tissues of hydrangea plants, and a transient expression study indicated that the gene product is localized to the plasma membrane. The results of an aluminum tolerance assay using yeast cells showed that the H m PALT 2 is also involved in the transport of other metal(loid)s. The over‐expression of HmPALT2 in A rabidopsis resulted in aluminum‐hypersensitivity, suggesting that H m PALT 2 should work as an aluminum transporter into cells in planta .