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Influence of radiation on Hemarthria compressa 's genetic variations
Author(s) -
Wu Bingchao,
Zhang Yu,
Yan Haidong,
Yang Shengting,
Zhou Sifan,
Zhang Xinquan,
Liu Wei,
Nie Gang,
Peng Yan,
Ma Xiao,
Yan Yanhong,
Li Zhou,
Huang Linkai
Publication year - 2018
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/grs.12203
Subject(s) - biology , mutant , genetic similarity , polymorphism (computer science) , primer (cosmetics) , genetics , genetic variation , botany , gene , genetic diversity , allele , chemistry , population , demography , organic chemistry , sociology
Using the material of Hemarthria compressa (L.F.) R.Br. cv. YA ’ AN , we carried out this research to study the influence of radiation on the genetic variation of plants. Genetic difference was analyzed with expressed sequence tag‐simple sequence repeat (EST‐SSR) molecular marker through the comparison of 60 Co‐ γ radiation on H. compressa seed stems and original variety. By using 20 primer pairs, 176 polymerase chain reaction (PCR)‐amplifications with clear and consistent bands were obtained. The results showed that 155 of 176 bands were polymorphic, which indicating an 88.07% polymorphism rate, and each pair of primers had 8.8 amplified bands on average; the amplitude of polymorphism information content was 0.4709–0.6952 with an average value 0.6081. The genetic similarity coefficient of H. compressa and its mutants ranged from 0.4318 to 0.8239 with an average of 0.6671. As a consequence, existence of genetic differences between the mutants and the basic material was proved.