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Generation and release of molecular markers for P oa arachnifera T orr.
Author(s) -
Kindiger Bryan,
Conley Terry,
Cai Hongwei
Publication year - 2013
Publication title -
grassland science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.388
H-Index - 19
eISSN - 1744-697X
pISSN - 1744-6961
DOI - 10.1111/grs.12021
Subject(s) - microsatellite , molecular marker , biology , genetic marker , primer (cosmetics) , genetics , genomic dna , molecular breeding , botany , computational biology , dna , gene , allele , chemistry , organic chemistry
DNA ‐based molecular markers can be used in a wide array of plant genetic studies, marker‐trait associations, seed purity evaluations and cultivar protection. However, for the genus P oa , the use of molecular markers is limited by the current lack of informative DNA based markers. This report represents the development and release of an additional 39 molecular markers, all derived from an unenriched P oa arachnifera Torr. genomic library. The newly released markers include genomic microsatellite markers, tandem repeats, palindromes and regions exhibiting low complexity generated from the P . arachnifera library. Many of the primer pairs used to amplify the markers were observed to exhibit polymorphisms when evaluated across five additional P oa species ( P. pratensis L., P. secunda J. Presl, P. ligularis Nees ex Steud., P. poiformis [Labill.] Druce and P. labillardieri Steud.) The availability of these new and informative markers should provide P oa breeders and geneticists with additional tools for breeding and genetic research.