Premium
Neutrophil CD 64 level as a rapid and promising diagnostic tool for infectious diseases in elderly patients
Author(s) -
Otsuki Nozomi,
Tsutani Hiroshi,
Matsui Toshihiro,
Iwasaki Hiromichi,
Ueda Takanori
Publication year - 2016
Publication title -
geriatrics and gerontology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 57
eISSN - 1447-0594
pISSN - 1444-1586
DOI - 10.1111/ggi.12470
Subject(s) - medicine , cd64 , infectious disease (medical specialty) , receiver operating characteristic , absolute neutrophil count , immunology , disease , flow cytometry , gastroenterology , neutropenia , toxicity
Aim We examined the utility of the neutrophil CD 64 level as a rapid and sensitive diagnostic marker for infections in febrile aged patients. Methods The expression level of CD 64 per neutrophil was quantitatively measured with flow cytometry using a QuantiBrite kit in samples from febrile (aged >65 years) patients. Information about the presence or absence of infectious disease was retrospectively obtained from each patient's medical record in which attending physicians were obliged to write down a tentative diagnosis after resolution of manifestations. Results With receiver operating characteristic curve evaluation using the results, a CD 64 level >2000 molecules per neutrophil was sensitive and specific for detecting infection. Among 102 patients suspected of having infection, 72 patients were diagnosed with infectious diseases, and 30 patients had non‐infectious diseases. The sensitivity and specificity of determination of the neutrophil CD 64 level were 88% and 63%, respectively. However, considering the high frequency of infections in elderly patients (71% in the present study), the post‐test probability reached as high as 93%. The positive likelihood ratio was 2.4, and the negative likelihood ratio was 0.2. Conclusions Considering the frequency of infectious diseases in elderly patients, determination of the neutrophil CD64 level helps detect infectious diseases. Geriatr Gerontol Int 2015; 16: 307–313.