Premium
Blueberry treatment attenuates D ‐galactose‐induced oxidative stress and tissue damage in rat liver
Author(s) -
Çoban Jale,
BetülKalaz Esra,
Küçükgergin Canan,
Aydın A Fatih,
DoğanEkici Işın,
DoğruAbbasoğlu Semra,
Uysal Müjdat
Publication year - 2014
Publication title -
geriatrics and gerontology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 57
eISSN - 1447-0594
pISSN - 1444-1586
DOI - 10.1111/ggi.12096
Subject(s) - gpx1 , sod2 , oxidative stress , glutathione peroxidase , superoxide dismutase , glutathione , malondialdehyde , gpx4 , endocrinology , medicine , chemistry , antioxidant , phospholipid hydroperoxide glutathione peroxidase , sod1 , biochemistry , enzyme
Aim d ‐galactose ( GAL ) causes aging‐related changes and oxidative stress in the organism. We investigated the effect of whole fresh blueberry ( BB ; V accinium corymbosum L .) treatment on oxidative stress in age‐related liver injury model. Methods Rats received GAL (300 mg/kg, s.c.; 5 days per week) alone or together with 5% ( BB 1) and 10% ( BB 2) BB ‐containing chow for 2 months. Serum alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities, and hepatic malondialdehyde ( MDA ), protein carbonyl ( PC ) and glutathione ( GSH ) levels, and CuZn ‐superoxide dismutase ( SOD 1), glutathione peroxidase ( GPx 1) and glutathione transferase ( GST ) activities together with mRNA expressions of SOD 1, GPx 1, MnSOD ( SOD 2) and phospholipid hydroperoxide glutathione peroxidase ( GPx 4) were determined in GAL ‐treated rats. Results MDA and PC levels increased, but GSH levels, SOD 1 and GPx 1 activities decreased together with histopathological structural damage in the liver in GAL ‐treated rats. There was no change in hepatic mRNA expressions of SOD 2 and GPx 1, but SOD 1 and GPx 4 expressions decreased. BB 1 and BB 2 caused significant decreases in serum ALT and AST activities together with the amelioration in histopathological findings in GAL ‐treated rats. Both BB 1 and BB 2 reduced MDA and PC levels, and elevated GSH levels, and SOD 1 and GPx 1 activities. However, hepatic mRNA expressions of SOD 1, SOD 2, GPx 1 and GPx 4 remained unchanged in GAL ‐treated rats. Conclusions These results show that BB restored liver pro‐oxidant status together with histopathological amelioration by acting as an anti‐oxidant (radical scavenger) itself without affecting mRNA expressions of anti‐oxidant enzymes in GAL ‐treated rats. Geriatr Gerontol Int 2014; 14: 490–497.