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The effects of lactic acid bacteria strains isolated from various substrates on the fermentation quality of common vetch ( Vicia sativa L.) in Tibet
Author(s) -
Wang Siran,
Li Junfeng,
Dong Zhihao,
Chen Lei,
Yuan Xianjun,
Shao Tao
Publication year - 2018
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/gfs.12363
Subject(s) - vicia sativa , silage , microbial inoculant , lactic acid , fermentation , lactobacillus plantarum , food science , starter , acetic acid , bacteria , biology , chemistry , horticulture , zoology , biochemistry , genetics
Eight lactic acid bacteria (LAB) strains isolated from five naturally fermented silages of different ensilage materials on the Tibetan Plateau were characterized, and their effects on the silage quality of common vetch ( Vicia sativa L.) were studied. These LAB isolates were evaluated using morphological, physiological and biochemical tests. The eight isolated strains and one commercial inoculant ( Lactobacillus plantarum MTD‐1) were subsequently added to common vetch for ensiling 60 days. All the isolated strains (LW4, M1, WG27, O30, I2, LCG3, LCG9, CG35) could grow normally at 5–20°C, pH 3.5–6.0 and NaCl (3.0%, 6.5%), and they were identified as Lactobacillus plantarum and Pediococcus pentosaceus by sequencing 16S rDNA. All the LAB inoculants improved the silage quality of common vetch, indicated by significantly ( p < .05) higher lactic acid (LA) contents and ratios of lactic acid to acetic acid (LA/AA), and significantly ( p < .05) lower pH and ammonia nitrogen (NH 3 –N) contents. Strain LCG3 performed best among all LAB inoculants, indicated by the highest ( p < .05) LA content and ratio of LA/AA, and the lowest ( p < .05) pH and NH 3 –N content. Strain LCG3 is recommended as starter culture for common vetch silage.