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Impact of ferulic acid esterase‐producing lactobacilli and fibrolytic enzymes on ensiling and digestion kinetics of mixed small‐grain silage
Author(s) -
Jin L.,
Dunière L.,
Lynch J. P.,
Zaheer R.,
Turkington K.,
Blackshaw R. E.,
Lupwayi N. Z.,
O'Donovan J. T.,
Harker K. N.,
McAllister T.,
Baah J.,
Wang Y.
Publication year - 2017
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/gfs.12217
Subject(s) - silage , microbial inoculant , lactobacillus buchneri , chemistry , agronomy , food science , fermentation , lactic acid , biology , zoology , inoculation , lactobacillus plantarum , horticulture , bacteria , genetics
This study evaluated the effects of a ferulic acid esterase ( FAE ) and a non‐ FAE ‐producing inoculant applied alone or in combination with exogenous fibrolytic enzymes ( EFE ) on the fermentation and nutritive value of mixed grain (barley, oats and spring triticale) silage. The mixed crop was ensiled in laboratory mini‐silos either untreated ( CON ), or treated with a FAE inoculant ( FAE ), a non‐ FAE inoculant ( NFAE ) or NFAE + EFE . Inoculated silages were lower ( P < 0·01) in water‐soluble carbohydrate, whereas NFAE and NFAE + EFE silages had higher ( P < 0·001) DM loss than other silages. FAE and NFAE silage had higher neutral detergent fibre ( NDF ), but were lower in NFAE + EFE than other silages ( P < 0·001). Copy numbers of 16S r RNA associated with Lactobacillus buchneri were higher ( P < 0·001) in NFAE and NFAE + EFE silages than in others, resulting in higher ( P < 0·001) acetic acid in these silages. NFAE + EFE silage had lower ( P < 0·001) in vitro gas production and NDF digestibility ( NDFD ) than other silages. FAE silage had higher ( P < 0·01) in situ NDFD than CON and NFAE + EFE silages. Inoculation of mixed small‐grain silage with NFAE ‐producing inoculants combining EFE reduced NDFD .