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Diversity of condensed tannin structures affects rumen in vitro methane production in sainfoin ( Onobrychis viciifolia ) accessions
Author(s) -
Hatew B.,
Hayot Carbonero C.,
Stringano E.,
Sales L. F.,
Smith L. M. J.,
MuellerHarvey I.,
Hendriks W. H.,
Pellikaan W. F.
Publication year - 2015
Publication title -
grass and forage science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.716
H-Index - 56
eISSN - 1365-2494
pISSN - 0142-5242
DOI - 10.1111/gfs.12125
Subject(s) - rumen , tannin , proanthocyanidin , condensed tannin , forage , fermentation , peg ratio , legume , temperate climate , zoology , chemistry , principal component analysis , biology , horticulture , food science , agronomy , botany , mathematics , biochemistry , finance , economics , polyphenol , statistics , antioxidant
Abstract Sainfoin is a non‐bloating temperate forage legume with a moderate‐to‐high condensed tannin ( CT ) content. This study investigated whether the diversity of sainfoin accessions in terms of CT structures and contents could be related to rumen in vitro gas and methane ( CH 4 ) production and fermentation characteristics. The aim was to identify promising accessions for future investigations. Accessions differed ( P < 0·0001) in terms of total gas and CH 4 productions. Fermentation kinetics (i.e. parameters describing the shape of the gas production curve and half‐time gas production) for CH 4 production were influenced by accession ( P ≤ 0·038), but not by PEG . Accession, PEG and time affected ( P < 0·001) CH 4 production, but accession and PEG interaction showed only a tendency ( P = 0·08). Increase in CH 4 due to PEG addition was not related to CT content. Further analysis of the relationships among multiple traits (nutritional composition, CT structure and CH 4 production) using principal component analysis ( PCA ) based on optimally weighted variables revealed differences among accessions. The first two principal component axes, PC 1 (57·6%) and PC 2 (18·4%), explained 76·0% of the total variation among accessions. Loading of biplots derived from both PCA s made it possible to establish a relationship between the ratio of prodelphinidin:procyanidin ( PD : PC ) tannins and CH 4 production in some accessions. The PD : PC ratio seems to be an important source of variation that is negatively related to CH 4 production. These results suggested that sainfoin accessions collected from across the world exhibited substantial variation in terms of their effects on rumen in vitro CH 4 production, revealing some promising accessions for future investigations.