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In vitro and in vivo removal of oral Candida from the denture base
Author(s) -
Kawasaki Kiyotsugu,
Kamikawa Yoshiaki,
Sugihara Kazumasa
Publication year - 2016
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/ger.12149
Subject(s) - candida albicans , candida glabrata , contamination , medicine , in vivo , candida infections , acrylic resin , dentistry , saline , scanning electron microscope , microbiology and biotechnology , materials science , composite material , biology , ecology , coating
Objectives To clarify the effectiveness of ultrasonic cleaning for removing Candida lodged in the denture base. Materials and Methods In vitro – Specimens of acrylic resin for denture plates contaminated with Candida cells were ultrasonically cleaned for 30 min. Washings were sampled every 5 min and cultured to investigate residual contamination, measured as colony forming units/ml, and the surfaces of the specimens were subjected to low‐vacuum scanning electron microscopy ( LV ‐ SEM ). In vivo – A total of 24 maxillary denture bases were brushed for 2 min under running tap water, then ultrasonically cleaned for 30 min. The washings were sampled every 5 min and cultured to investigate residual contamination. Results In vitro – Maximum removal was achieved during the first 5 min of cleaning, with the mean CFU /ml counts significantly declining over time. More than 85% of all Candida was removed within the first 15 min in specimens inoculated with phosphate‐buffered saline suspensions of Candida albicans and Candida glabrata . LV ‐ SEM revealed a decreased number of Candida on the surface of the specimens after 30 min of ultrasonic cleaning. In vivo – Maximum removal was achieved during the first 5 min of cleaning, then the mean CFU /ml count significantly declined during the first 10 min. Ultrasonic cleaning for 15 min removed 88.4% of Candida species from the denture base. Conclusions Ultrasonic cleaning is a reliable and simple method for removing Candida lodged in the denture base.