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Effectiveness of three antimicrobial mouthrinses on the disinfection of toothbrushes stored in closed containers: a randomized clinical investigation by DNA C heckerboard and C ulture
Author(s) -
Nascimento Cássio do,
Sorgini Maíra Balero,
Pita Murillo Sucena,
Fernandes Flávio Henrique Carriço Nogueira,
Calefi Paulo Linares,
Watanabe Evandro,
Pedrazzi Vinícius
Publication year - 2014
Publication title -
gerodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.7
H-Index - 54
eISSN - 1741-2358
pISSN - 0734-0664
DOI - 10.1111/ger.12035
Subject(s) - checkerboard , medicine , dentistry , toothbrush , chlorhexidine , antimicrobial , contamination , negative control , microbiology and biotechnology , traditional medicine , biology , ecology , brush , electrical engineering , engineering
Objectives The aim of this in vivo study was to evaluate the efficacy of three antimicrobial solutions on the disinfection of toothbrushes after storage in closed containers. Materials and Methods Sixteen healthy subjects were enroled in this randomized cross‐over clinical investigation. The study was conducted in four phases, in which mouthrinses (chlorhexidine gluconate‐based or cetilpiridinium‐based) and sterile tap water (control group) were used to individually store used toothbrushes in closed containers during 7 days of toothbrushing. Five toothbrushes were used as negative control for bacterial colonisation before contact with oral cavity. Conventional culture and DNA Checkerboard hybridization were used to detect bacterial contamination on the toothbrushes. Subsequently, the number of bacterial species on the bristles was estimated by the DNA Checkerboard method. Results One toothbrush presented bacterial contamination in the negative control test. Both culture and DNA Checkerboard showed positive signals of bacterial contamination in the toothbrushes with no differences in the frequency of detection. The control group showed higher total bacterial counts when compared with the mouthrinse groups. Porphyromonas gingivalis had the highest bacterial count followed by P arvimonas micra . Conclusion Culture and DNA Checkerboard showed positive signals of bacterial contamination. Mouthrinses that contains 0.12% of chlorhexidine gluconate were more effective in reducing bacterial colonisation on the toothbrushes.