Open AccessGenotypic variation of cell wall composition and its conversion efficiency in Miscanthus sinensis , a potential biomass feedstock crop in China
Author(s) -
Zhao Hua,
Li Qian,
He Junrong,
Yu Jiangyan,
Yang Junpin,
Liu Chunzhao,
Peng Junhua
Publication year - 2014
Publication title -
gcb bioenergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.378
H-Index - 63
eISSN - 1757-1707
pISSN - 1757-1693
DOI - 10.1111/gcbb.12115
Subject(s) - hemicellulose , cellulose , lignin , miscanthus , miscanthus sinensis , biomass (ecology) , bioenergy , raw material , secondary cell wall , biohydrogen , agronomy , botany , food science , chemistry , biology , biofuel , microbiology and biotechnology , biochemistry , ecology , hydrogen production , catalysis
Plant cell walls are composed of cellulose microfibrils embedded in a cross‐linked‐net of matrix polysaccharides and co‐polymerized with lignin. The study presented the genotypic variations of cell wall composition, biohydrogen production, and lignocellulose degradation ratio in a collection of 102 Miscanthus sinensis ( M . Sinensis , hereafter) accessions collected from a wide geographical range in China. Significant variations were observed for the determined traits, cellulose content, hemicellulose content, cellulose and hemicellulose degradation efficiency, and biohydrogen yield. The cellulose, hemicellulose, and lignin contents ranged from 30.20–44.25, 28.97–42.65, and 6.96–20.75%, respectively. The degradation ratio of cellulose and hemicellulose varied from 2.08% to 37.87% and from 14.71% to 52.50%, respectively. The feedstock was fermented to produce biohydrogen, and the production varied from 14.59 to 40.66 ml per gram of Miscanthus biomass. The expression profile of three cellulose synthase ( MsCesA ) genes was initially established to indicate the genotypic difference among the M . sinensis accessions. Pearson's correlations were conducted to reveal the perplexing relationship between the tested traits, biohydrogen yield, cell wall composition and their degradation efficiency. In addition, the relationship pattern, between the test traits and the geographic factors corresponding with the original place, was investigated. The result showed that the significant variation among the M . sinensis genotypes is the result of natural selection in different environments of their original habitats. Improvement in cell wall composition and structure and enhancement of lignocellulose degradation ratio could significantly increase sustainable bioenergy production.