Open AccessEnzymatic saccharification of cornstalk by onsite cellulases produced by Trichoderma viride for enhanced biohydrogen production
Author(s) -
Zhao Lei,
Cao GuangLi,
Wang AiJie,
Ren HongYu,
Xu ChengJiao,
Ren NanQi
Publication year - 2013
Publication title -
gcb bioenergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.378
H-Index - 63
eISSN - 1757-1707
pISSN - 1757-1693
DOI - 10.1111/gcbb.12022
Subject(s) - biohydrogen , trichoderma viride , chemistry , cellulase , hydrolysate , enzymatic hydrolysis , lignocellulosic biomass , fermentation , cellulosic ethanol , cellulose , pulp and paper industry , hydrolysis , biomass (ecology) , raw material , food science , biochemistry , hydrogen production , agronomy , biology , organic chemistry , engineering , catalysis
Lignocellulosic biomass, if properly saccharified, could be an ideal feedstock for biohydrogen production. However, the high cellulases cost is the key obstacle to its development. In this work, cost‐effective enzyme produced by Trichoderma viride was used to saccharify cornstalk. To obtain high sugar yield, a central composite design of response surface method was used to optimize enzymatic saccharification process. Experimental results showed that the enzymatic saccharification rate reached the highest of 81.2% when pH, temperature, cellulases and substrate concentration were 5, 49.7 °C, 35.7 IU g −1 , and 38.5 g L −1 , respectively. The cornstalk hydrolysate was subsequently introduced to fermentation by Thermoanaerobacterium thermosaccharolyticum W16, the yield of hydrogen reached the highest level of 90.6 ml H 2 g −1 pretreated cornstalk. The present results indicate the potential of using T. thermosaccharolyticum W16 for high yield conversion of cornstalk hydrolysate, which was saccharified by onsite enzyme produced by T. viride .