Premium
Lipofuscin labeling through biorthogonal strain‐promoted azide‐alkyne cycloaddition for the detection of senescent cells
Author(s) -
LozanoTorres Beatriz,
Blandez Juan F.,
GarcíaFernández Alba,
Sancenón Félix,
MartínezMáñez Ramón
Publication year - 2023
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.16477
Subject(s) - fluorophore , azide , chemistry , moiety , cycloaddition , bodipy , lipofuscin , fluorescence , strain (injury) , biochemistry , stereochemistry , biology , organic chemistry , anatomy , physics , quantum mechanics , catalysis
A new method for senescent cell detection is described, which is based on lipofuscin labeling with a fluorescent reporter through a biorthogonal strain‐promoted azide‐alkyne cycloaddition. The sensing protocol involves a first step where the interaction of lipofuscin with a Sudan Black B derivative containing an azide moiety ( SBB‐N 3 ) is carried out. In the final step, the azide moiety reacts with a fluorophore containing a cyclooctene ring ( BODIPY ). The efficacy of this two‐step protocol is assessed in senescent melanoma SK‐MEL‐103 cells, senescent triple‐negative breast cancer MDA‐MB‐231 cells and senescent WI‐38 fibroblasts. In all cases, a clear fluorescence pattern was observed in senescent cells, compared to proliferative cells, only when the SBB‐N 3 ‐BODIPY probe was formed. Our results provide an alternative tool for the detection of senescent cells, based on an in situ bio‐orthogonal reaction for lipofuscin labeling.