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Chromatin accessibility of kidney tubular cells under stress reveals key transcription factor mediating acute and chronic kidney disease
Author(s) -
Xing Yuexian,
Wang Qi,
Zhang Jing,
Li Wenju,
Duan Aiping,
Yang Jingping,
Liu Zhihong
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15818
Subject(s) - transcription factor , chromatin , reprogramming , biology , hnf1b , kidney , microbiology and biotechnology , kidney disease , cancer research , gene , genetics , endocrinology , homeobox
Cellular injury caused by stimuli plays an important role in the progression of various diseases including acute and chronic kidney diseases. The dynamic transcriptional regulation responding to stimuli underlies the important mechanism of injury. In this study, we investigated the regulatory elements and their dynamic activities in kidney tubular epithelial cells. We captured the chromatin accessibility and gene expression with ATAC‐seq and RNA sequencing under a variety of extracellular stimuli including H 2 O 2 , TGF‐β1, and FG4592 which is an agonist of hypoxia‐inducible factor. Our results revealed both condition‐specific and condition‐shared transcription regulation. Interestingly, the shared regulation program revealed that the key transcription factor HNF1B‐mediated cellular reprogramming leads to a common change among the stimuli. We found the HNF1B regulatory network was significantly disrupted in various kidney diseases.