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Biological functions, genetic and biochemical characterization, and NMR structure determination of the small zinc finger protein HVO_2753 from Haloferax volcanii
Author(s) -
Zahn Sebastian,
Kubatova Nina,
Pyper Dennis J.,
Cassidy Liam,
Saxena Krishna,
Tholey Andreas,
Schwalbe Harald,
Soppa Jörg
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15559
Subject(s) - haloferax volcanii , chemistry , cysteine , mutant , complementation , zinc , escherichia coli , zinc finger , biochemistry , stereochemistry , gene , archaea , organic chemistry , enzyme , transcription factor
A zinc finger µ‐protein of the halophilic archaeon Haloferax volcanii , HVO_2753, is essential for swarming and biofilm formation. It contains four C(P)XCG motifs and, thus, two putative zinc binding pockets (ZBP). The NMR solution structure was solved, and, unexpectedly, revealed that only ZBP2 contains a zinc ion, while ZBP1 is not occupied.