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Sarcolemma wounding activates dynamin‐dependent endocytosis in striated muscle
Author(s) -
McDade Joel R.,
Naylor Molly T.,
Michele Daniel E.
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15556
Subject(s) - dynamin , sarcolemma , dysferlin , endocytosis , microbiology and biotechnology , skeletal muscle , chemistry , myocyte , biology , biochemistry , anatomy , cell
Dysferlin‐deficient muscle fibers show elevated uptake of the extracellular dye, FM1‐43, following wounding, which is the basis for the assertion that membrane repair is defective in this model. We show that the wound‐induced uptake of FM1‐43 occurs via dynamin‐dependent endocytosis. Further, dynamin inhibition mitigates the differences in wound‐induced FM1‐43 uptake between dysferlin‐null and wild‐type muscle fibers, suggesting that wounding may result in overactive dynamin‐dependent endocytosis in dysferlin‐deficient muscle fibers.