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Headless C1q: a new molecular tool to decipher its collagen‐like functions
Author(s) -
Fouët Guillaume,
Bally Isabelle,
Signor Luca,
Häußermann Katharina,
Thielens Nicole M.,
Rossi Véronique,
Gaboriaud Christine
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15543
Subject(s) - tetramer , complement system , recombinant dna , triple helix , chemistry , classical complement pathway , biophysics , complement c1q , microbiology and biotechnology , biochemistry , biology , stereochemistry , immune system , genetics , gene , enzyme
Replacement of C1q globular regions (GR) by type IX collagen nc2 domains allows the recombinant production of well‐assembled and folded C1q collagen‐like regions (CLR). This represents the first reported production of a chimeric C1q devoid of its GR functions while retaining CLR properties. Such development is of great interest and opens the way to molecular investigations of CLRs major functions such as complement activation and immune modulation.