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A guide to: generation and design of nanobodies
Author(s) -
Muyldermans Serge
Publication year - 2021
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15515
Subject(s) - computational biology , heavy chain , variety (cybernetics) , domain (mathematical analysis) , computer science , trademark , antibody , biology , immunology , mathematics , artificial intelligence , mathematical analysis , operating system
Immune or naïve nanobody (Nb) repertoires are cloned from B lymphocytes of camelids. Alternatively, synthetic Nb libraries are generated in vitro by randomising codons forming antigen‐binding loops of a Nb scaffold. These libraries serve to retrieve target‐specific Nbs by phage display pannings, yeast surface display or other selection methods. After identification of the lead Nb and possible engineering efforts, multiple applications have been explored in research, diagnosis or therapy.