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More precise, more universal and more specific – the next generation of RNA‐guided endonucleases for genome editing
Author(s) -
Marzec Marek,
Hensel Goetz
Publication year - 2019
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.15079
Subject(s) - crispr , cleave , genome editing , computational biology , rna , genome , dna , biology , genetics , endonuclease , gene
CRISPR is a prokaryotic defence system that was adapted as a tool for genome editing and has become one of the most important discoveries of this century. CRISPR‐associated endonucleases cleave DNA at precise sites, which are marked by complementary short‐guided RNA. The recently developed versions of endonucleases are compatible with a broad range of PAM motifs, have a higher specificity and enable a specific nucleotide to be replaced.