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The GRASP domain in golgi reassembly and stacking proteins: differences and similarities between lower and higher Eukaryotes
Author(s) -
Mendes Luís F. S.,
Fontatália A.,
Oliveira Carolina G.,
Freire Marjorie C. L. C,
Lopes José L. S.,
Melo Fernando A.,
CostaFilho Antonio J.
Publication year - 2019
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14869
Subject(s) - golgi apparatus , microbiology and biotechnology , secretory pathway , biology , endoplasmic reticulum
The Golgi complex is part of the endomembrane system and is responsible for receiving transport cargos from the endoplasmic reticulum and for sorting and targeting them to their final destination. To perform its function in higher eukaryotic cells, the Golgi needs to be correctly assembled as a flattened membrane sandwich kept together by a protein matrix. The precise mechanism controlling the Golgi cisternae assembly is not yet known, but it is widely accepted that the Golgi Reassembly and Stacking Protein ( GRASP ) is a main component of the Golgi protein matrix. Unlike mammalian cells, which have two GRASP genes, lower eukaryotes present only one gene and distinct Golgi cisternae assembly. In this study, we performed a set of biophysical studies to get insights on the structural properties of the GRASP domains ( DGRASP s) from both human GRASP 55 and GRASP 65 and compare them with GRASP domains from lower eukaryotes ( Saccharomyces cerevisiae and Cryptococcus neoformans ). Our data suggest that both human DGRASP s are essentially different from each other and that DGRASP 65 is more similar to the subgroup of DGRASP s from lower eukaryotes in terms of its biophysical properties. GRASP 55 is present mainly in the Golgi medial and trans faces, which are absent in both fungi, while GRASP 65 is located in the cis ‐Golgi. We suggest that the GRASP 65 gene is more ancient and that its paralogue GRASP 55 might have appeared later in evolution, together with the medial and trans Golgi faces in mammalians.