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Rv0474 is a copper‐responsive transcriptional regulator that negatively regulates expression of RNA polymerase β subunit in Mycobacterium tuberculosis
Author(s) -
Raghunandanan Sajith,
Ramachandran Ranjit,
Gomez Roshna Lawrence,
Devanarayanan Sivasankar,
Bommakanti Akhila,
Kondapi Anand Kumar,
Varadarajan Raghavan,
Kumar Ramakrishnan Ajay
Publication year - 2018
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14637
Subject(s) - rpob , psychological repression , mycobacterium tuberculosis , rna polymerase , transcriptional regulation , leucine zipper , protein subunit , promoter , regulator , biology , microbiology and biotechnology , chemistry , rna , bacteria , biochemistry , gene , transcription factor , genetics , tuberculosis , gene expression , medicine , 16s ribosomal rna , pathology
We characterize Rv0474, a putative transcriptional regulatory protein of Mycobacterium tuberculosis , which is found to function as a copper‐responsive transcriptional regulator at toxic levels of copper. It is an autorepressor, but at elevated levels (10–250 μ m ) of copper ions the repression is relieved resulting in an increase in Rv0474 expression. Copper‐bound Rv0474 is recruited to the rpoB promoter leading to its repression resulting in the growth arrest of the bacterium. Mutational analysis showed that the helix‐turn‐helix and leucine zipper domains of Rv0474 are essential for its binding to Rv0474 and rpoB promoters, respectively. The mechanism of Rv0474‐mediated rpoB regulation seems to be operational only in pathogenic mycobacteria that can persist inside the host.