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Arf1 regulates the ER –mitochondria encounter structure ( ERMES ) in a reactive oxygen species‐dependent manner
Author(s) -
Zhang Bing,
Yu Qilin,
Huo Da,
Li Jianrong,
Liang Chao,
Li Hongyue,
Yi Xiao,
Xiao Chenpeng,
Zhang Dan,
Li Mingchun
Publication year - 2018
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14445
Subject(s) - microbiology and biotechnology , endoplasmic reticulum , intracellular , golgi apparatus , reactive oxygen species , mitochondrion , chemistry , candida albicans , biology
The Arf family of small GTP ‐binding and ‐hydrolyzing proteins are some of the most important intracellular regulators of membrane dynamics. In this study, we identified the Golgi‐localized Arf family G protein Arf1 in Candida albicans and confirmed its conserved function in regulating the secretory pathway. Interestingly, deletion of ARF 1 resulted in intracellular reactive oxygen species ( ROS ) accumulation, and induced formation of the endoplasmic reticulum ( ER )–mitochondria encounter structure ( ERMES ). Moreover, N ‐acetylcysteine‐mediated ROS scavenging in the arf1 Δ/Δ strain attenuated ERMES formation, indicating that intracellular ROS accumulation resulting from ARF 1 deletion facilitated ERMES formation. In addition, Arf1 regulated many key physiological processes in C. albicans , including cell cycle progression, morphogenesis and virulence. This study uncovers a role for Arf family G proteins in regulating ERMES formation and sheds new light on the crucial contribution of ROS to membrane dynamics.