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Pushing the haystack aside for efficient gene targeting in human cells
Author(s) -
Matsumoto Yoshihisa
Publication year - 2017
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14164
Subject(s) - haystack , homologous recombination , gene targeting , gene , computational biology , biology , function (biology) , genetics , computer science , world wide web
Gene targeting, i.e., the disruption of a specific gene via homologous recombination, is one of the key techniques to study gene function. However, researchers have faced the difficulty that, in human cells, the frequency of correct targeted integration is quite low compared to non‐targeted (or random) integration. Adachi and colleagues investigated the factors that influence the ratio of targeted integration to non‐targeted integration.