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Real‐time crystallographic studies of the adenine riboswitch using an X‐ray free‐electron laser
Author(s) -
Stagno Jason R.,
Bhandari Yuba R.,
Conrad Chelsie E.,
Liu Yu,
Wang YunXing
Publication year - 2017
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14110
Subject(s) - femtosecond , riboswitch , crystallography , aptamer , chemistry , laser , electron crystallography , rna , physics , electron diffraction , optics , diffraction , biology , biochemistry , genetics , gene , non coding rna
Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X‐ray free‐electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix‐and‐inject crystallography. These results illustrate the structural basis for the ligand‐induced conformational changes associated with the molecular ‘switch’.