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AID ing‐targeted protein degradation in Drosophila
Author(s) -
Zhang Xu,
Schnorrer Frank
Publication year - 2017
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14054
Subject(s) - protein degradation , microbiology and biotechnology , drosophila (subgenus) , degradation (telecommunications) , biology , computational biology , chemistry , computer science , biochemistry , gene , telecommunications
Conditional protein depletion is highly desirable for investigating protein functions in complex organisms. In this issue, Bence and colleagues combined auxin‐inducible degradation with CRISPR, establishing an elegant tool to control protein levels. They achieve precise spatio‐temporal control of protein degradation during Drosophila oogenesis and early embryogenesis by combining suitable GAL4 drivers (spatial control) with auxin feeding protocols (temporal control).