Premium
Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage
Author(s) -
Chen Wen,
Jiang Youzhao,
Han Jian,
Hu Jiongyu,
He Ting,
Yan Tiantian,
Huang Na,
Zhang Qiong,
Mei Hao,
Liao Yong,
Huang Yuesheng,
Chen Bing
Publication year - 2017
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.14038
Subject(s) - adipose triglyceride lipase , podocyte , lipid droplet , medicine , endocrinology , lipotoxicity , lipid metabolism , chemistry , kidney , microbiology and biotechnology , lipolysis , biology , adipose tissue , insulin , insulin resistance , proteinuria
Abnormal lipid metabolism, renal lipid accumulation and lipotoxicity are associated with the pathological features of glomerulopathy. However, the mechanisms by which lipid accumulation leads to the development or progression of this disease have not been fully elucidated. In this work, we have identified a role for the rate‐limiting enzyme in lipolysis, adipose triglyceride lipase ( ATGL ; also called patatin‐like phospholipase domain‐containing protein 2), in renal lipid metabolism and kidney disease. ATGL ‐deficient ( Atgl (−/−)) mice displayed albuminuria, accompanied by ectopic deposition of fat in the kidney. Magnetic resonance imaging demonstrated that the contrast agent gadopentetic acid was retained in kidney tissue, suggesting defects in the glomerular filtration barrier. Furthermore, transmission electron microscopy revealed lipid deposits in the podocyte, along with foot process fusion and morphological changes suggestive of apoptosis. Indeed, shRNA‐mediated depletion of ATGL promoted podocyte apoptosis, accompanied by increased levels of intracellular reactive oxygen species ( ROS ) and F‐actin fibre redistribution. These effects could be partially reversed by treatment with the antioxidant N ‐acetylcysteine. These data suggest that ATGL deficiency induces renal lipid accumulation, proteinuria and glomerular filtration barrier dysfunction and implicate increased intracellular ROS levels in inducing podocyte F‐actin rearrangement, foot process fusion and apoptosis that underlie these pathological features. Enzymes Adipose triglyceride lipase, EC 3.1.1.3.