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A regulatory subunit of protein phosphatase 2A, PPP 2R5E, regulates the abundance of microtubule crosslinking factor 1
Author(s) -
Hyodo Toshinori,
Ito Satoko,
AsanoInami Eri,
Chen Dan,
Senga Takeshi
Publication year - 2016
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13835
Subject(s) - microtubule , phosphatase , okadaic acid , protein subunit , protein phosphatase 2 , microbiology and biotechnology , protein phosphatase 1 , chemistry , microtubule associated protein , biology , biochemistry , phosphorylation , gene
Dynamic changes in microtubule organization are regulated by numerous microtubule‐associating proteins and their post‐translational modification. Microtubule crosslinking factor 1 ( MTCL 1) is a recently identified protein that regulates microtubule organization. To obtain further insight into its functions, we searched for proteins that associate with it using mass spectrometry analysis. We found that PPP 2R5E, a regulatory subunit of protein phosphatase 2A, interacted with MTCL 1. Depletion of PPP 2R5E reduced the abundance of MTCL 1 abundance, whereas exogenous expression of PPP 2R5E increased endogenous MTCL 1. Furthermore, inhibition of phosphatase activity by okadaic acid reduced MTCL 1, which was restored by the addition of the protease inhibitor MG 132. Finally, we show that cells depleted of PPP 2R5E and MTCL 1 exhibited defects in microtubule organization. Our results suggest that the PPP 2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL 1.