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Polarized trafficking of the sorting receptor Sor LA in neurons and MDCK cells
Author(s) -
Klinger Stine C.,
Højland Anne,
Jain Shweta,
Kjolby Mads,
Madsen Peder,
Svendsen Anna Dorst,
Olivecrona Gunilla,
Bonifacino Juan S.,
Nielsen Morten S.
Publication year - 2016
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13758
Subject(s) - microbiology and biotechnology , transcytosis , endosome , chemistry , receptor , retromer , hippocampal formation , biology , endocytosis , neuroscience , biochemistry , intracellular
The sorting receptor Sor LA is highly expressed in neurons and is also found in other polarized cells. The receptor has been reported to participate in the trafficking of several ligands, some of which are linked to human diseases, including the amyloid precursor protein, TrkB, and Lipoprotein Lipase (LpL). Despite this, only the trafficking in nonpolarized cells has been described so far. Due to the many differences between polarized and nonpolarized cells, we examined the localization and trafficking of Sor LA in epithelial Madin–Darby canine kidney ( MDCK ) cells and rat hippocampal neurons. We show that Sor LA is mainly found in sorting endosomes and on the basolateral surface of MDCK cells and in the somatodendritic domain of neurons. This polarized distribution of Sor LA respectively depends on an acidic cluster and an extended version of this cluster and involves the cellular adaptor complex AP ‐1. Furthermore, we show that Sor LA can mediate transcytosis across a tight cell layer.