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Transcribing RNA polymerase III observed by electron cryomicroscopy
Author(s) -
Hoffmann Niklas A.,
Jakobi Arjen J.,
Vorländer Matthias K.,
Sachse Carsten,
Müller Christoph W.
Publication year - 2016
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13732
Subject(s) - cryo electron microscopy , rna polymerase ii , rna polymerase , polymerase , processivity , rna polymerase iii , transcription factor ii f , chemistry , transcription (linguistics) , biophysics , microbiology and biotechnology , rna , physics , biology , biochemistry , dna , gene expression , gene , promoter , linguistics , philosophy
Electron cryomicroscopy reconstructions of elongating RNA polymerase (Pol) III at 3.9 Å resolution and of unbound Pol III (apo Pol III ) in two distinct conformations at 4.6 Å and 4.7 Å resolution allow the construction of complete atomic models of Pol III and provide new functional insights into the adaption of Pol III to fulfill its specific transcription tasks.